Introduction to Recombinant DNA

Methods in genetic engineering

Introduction to Recombinant DNA

  • Bacteriophages replicate through the lytic phase cycle and the phage genome is injected int...}

Agarose Gel Electrophoresis

Methods in genetic engineering

Agarose Gel Electrophoresis

  • Electrophoresis is a technique used to separate and purify macromolecules like nucleic acid...}

Types of Restriction Endonuclease

Methods in genetic engineering

Types of Restriction Endonuclease

  • To terminate limit enzymes interest, heat inactivation is a manner however for the enzymes...}

DNA Modifying Enzymes

Methods in genetic engineering

DNA Modifying Enzymes

  • Ligases are important enzymes required for physiological cellular methods including DNA rep...}

PCR(Polymerase Chain Reaction)

Methods in genetic engineering

PCR(Polymerase Chain Reaction)

  • Calf intestinal phosphatase is a dimeric glycoprotein isolated from bovine intestine. ...}

Vectors

Methods in genetic engineering

Vectors

  • The word “plasmid” was brought by ‘Joshua Lederberg’ in 1952 and is...}

Expression of Plasmid Vectors

Methods in genetic engineering

Expression of Plasmid Vectors

  • There is an array of plasmid vectors which can generate huge amounts of mRNA in vivo from c...}

Thermostable DNA Polymerase

Methods in genetic engineering

Thermostable DNA Polymerase

  • The binding and polymerization area is occupied through the carboxy terminus while the stro...}

Bacteriophage vectors

Methods in genetic engineering

Bacteriophage vectors

  • Phages play an important function in molecular biology as cloning vectors to insert DNA int...}

Lambda-Replacement Vector

Methods in genetic engineering

Lambda-Replacement Vector

  •  Phage is packaged in vitro with the aid of reducing on the cos site. The cosmid with...}

Yeast Artificial Chromosome (YACs)

Methods in genetic engineering

Yeast Artificial Chromosome (YACs)

  • The massive size of DNA may be inserted into YAC vectors, generally among 250kilobases to 4...}